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. 2018 Oct 1;215(10):2617–2635. doi: 10.1084/jem.20180300

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© 2018 Ito et al.

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Figure 3. — Impact of CLIP mutation on islet-infiltrating CD4 T cell populations specific for fusion peptides. (A) Representative examples of tetramer labeling of islet-infiltrating CD4 T cells from 9-wk-old control (C) or M98A/M98A (KI) mice. Two-color tetramer stains (PE and allophycocyanin) are shown for negative control CLIP, 6.9HIP, and 2.5HIP peptides as well as InsB13-21 and InsB12-20 peptides. (B) Summary of tetramer labeling data for islet-infiltrating CD4 T cells (% tetramer⁺ cells of CD4⁺ cells) from 8-wk-, 9-wk-, and 12-wk-old mice (n = 5–13 mice/group). (C and D) Flow-cytometric analysis of islet-infiltrating CD8⁺ T cells from 12-wk-old control (C) or M98A/M98A (KI) mice. Two-color tetramer stains (PE and allophycocyanin) with IGRP (8D) control peptide as well as IGRP206-214 and Ins15-23 peptides (n = 8 mice/group). All data represent two independent experiments. Statistical analyses were performed with the Mann-Whitney test; mean + SD are shown.