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. 2018 Oct 1;215(10):2617–2635. doi: 10.1084/jem.20180300

Figure 9.

Figure 9.

Point mutation in CLIP diminishes presentation of free peptides but does not impact presentation of full length antigens. (A) Control (C) or M98A/M98A (KI) mice were immunized with OVA or HEL protein. On day 8, CD4+ T cells from dLNs were cultured with DCs from WT NOD mice with or without the immunization protein. Each dot represents an individual mouse (n = 4 mice/group). (B and C) DCs from control (C) or M98A/M98A (KI) mice were cultured with CD4+ T cells harvested from dLNs of WT NOD mice immunized with OVA323-339 peptides. OVA protein or OVA323-339 peptide were added to the culture at a concentration of 234 nM (B; n = 5 samples/group) or at the indicated concentrations (C), respectively. (D) DCs from control mice (C) or M98A/M98A (KI) mice were cultured with CD4+ T cells harvested from dLNs of WT NOD mice immunized with HEL11-27 peptide. Indicated concentrations of HEL protein or HEL11-27 peptide were added to the culture. Each dot represents mean of duplicate samples (C and D). IFN-γ production was examined by ELISPOT. Data representative of two independent experiments. Statistical analyses were performed with the Mann-Whitney test. Mean + SD are shown (A and B).