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. 2018 Oct 1;215(10):2567–2585. doi: 10.1084/jem.20180628

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© 2018 Hernandez et al.

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Figure 6. — IRF9 is required for optimal control of viral infections. (A) WB confirms the efficiency of RNAi of IRF9 or MAVS in primary dermal fibroblasts. (B–F) Primary human dermal fibroblasts previously transfected with the indicated siRNA (negative control, IRF9, MAVS) were tested for control of HRV, RSV, and PIV. Cells were infected with HRV-A16 at MOI of 10 (B), RSV at MOI of 0.5 (C and D), or PIV3 at MOI of 0.1 (E and F). Relative HRV transcripts (B) were measured by qRT-PCR, and values were normalized to the siNeg control. Percentage of infected cells (C and E) and relative virus per infected cell (D and F) were measured by flow cytometric analysis of GFP⁺ cells. MFI of GFP⁺ cells in individual samples were normalized to negative control at 24 h (D and F). Shown are the mean ± SD of six (B–F) experiments. *, P < 0.05; **, P < 0.01, by Kruskal-Wallis test.