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. 2018 Oct 3;12(5):054108. doi: 10.1063/1.5050451

FIG. 8.

FIG. 8.

Western blot analysis of the possible binding target of the Tx-01 aptamer, BRCA2. (a) The protein size of the recombinant human BRCA2 and human serum albumin (HSA). [(b) and (c)] 0.5 μg of recombinant human BRCA2 and HSA proteins on the PVDF membrane and hybridized with Tx-01 and Fib2 aptamers, respectively. The arrow points to the protein size of recombinant human BRCA2. (d) PVDF without aptamer hybridization. [(e) and (f)] 20 μg of proteins from total cell lysates of NIH/3T3 and OVCAR8 cell lines were denatured at 55 °C and separated by 10% (b) and 6% (c) SDS-PAGE, respectively. Arrows indicate proteins of different molecular weight recognized by the Tx-01 aptamer. The leftmost lane is the molecular weight marker indicating the protein size. Protein marker (Cat. #PM007-0500, GeneDireX).