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. Author manuscript; available in PMC: 2018 Oct 3.
Published in final edited form as: J Allergy Clin Immunol. 2017 Feb 10;140(6):1651–1659.e1. doi: 10.1016/j.jaci.2016.12.974

FIG 1.

FIG 1

ebo mutation leads to truncation of the LRRC8A C-terminus while preserving protein expression. A, Sanger sequencing showing a 2-bp deletion (delTG) in exon 3 of Lrrc8a in ebo/ebo mice. B, Semiquantitative RT-PCR of Lrrc8a mRNA from splenocytes of WT and ebo/ebo mice. C, Immunoblot analysis of LRRC8A expression in splenocytes of WT and ebo/ebo mice using anti-LRRC8A791–808 antibody. D, Comparison of amino acid sequence 87 to 104 in LRRC8A and the corresponding sequences in LRRC8 family member homologues. Amino acids shared between LRRC8 family members and LRRC8A are highlighted in yellow. E, Flow cytometric analysis of intracellular LRRC8A expression in CD3+ T cells of CD4 Cre Lrrc8af/f and C57BL/6 WT control mice (left panel) and ebo/ebo and FVB/N WT control mice (right panel) using anti-LRRC8A87–104 antibody. Columns and bars represent means and SEMs. ns, Not significant. ***P < .001, Student t test.