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. Author manuscript; available in PMC: 2018 Oct 3.
Published in final edited form as: J Allergy Clin Immunol. 2017 Feb 10;140(6):1651–1659.e1. doi: 10.1016/j.jaci.2016.12.974

FIG 3.

FIG 3

ebo dramatically reduces VRAC activity. A, Solution scheme used for patch-clamp analysis. B and D, Current traces induced in splenic T cells (Fig 3B) and peritoneal macrophages (Fig 3D) from WT (left panels) and ebo/ebo (right lower panels) mice by the indicated voltage step protocol shown in right upper panels. The dashed line indicates 0 current. C and E, Current densities induced in splenic T cells (Fig 3C) and peritoneal macrophages (Fig 3E) from ebo/ebo, Lrrc8a−/−, and WT control mice by using a 220 mOsm hypotonic stimulus at −80 mV (left panels) or +80 mV (right panels). Results are representative of 8 to 12 cells pooled from 3 or more independent experiments by using 1 mouse per group in each experiment.