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T-cells and leukocytes infiltrate the stroma of puncture site metastases. (A) Immunochemistry of puncture site metastases in three different C57BL/6 mice showing H + E staining (top), anti-CD3 staining of T cells (middle), and anti-CD11b staining of leukocytes (bottom), all taken at 10× power. (B) In all four samples stained (100%) from different C57BL/6 mice there was evidence of intratumoral and/or tumor stroma infiltration of CD3+ cells and CD11b+ cells.
