Table 2.

Central composite design for 3 variables over 16 runs; experimental and predicted  %lipid and  %protein values are presented

Run	Factors			Responses
	NaCl (%)	Solvent: biomass (v/w)	Time (min)	Experimental values		Predicted values
	(X1)	(X2)	(X3)	Yield lipid (%)	Protein (%)	Yield lipid (%)	Protein (%)
1	10	5	20	4.89	29.22	4.65	29.37
2	10	5	50	3.25	38.34	3.62	40.90
3	10	15	20	6.09	43.17	6.39	46.43
4	10	15	50	3.92	19.86	3.67	18.14
5	30	5	20	2.52	22.61	2.91	26.91
6	30	5	50	3.54	46.94	3.37	46.26
7	30	15	20	2.67	31.25	2.44	31.26
8	30	15	50	0.82	8.38	1.19	10.81
9	20	10	35	6.01	42.38	6.02	43.45
10	2.36	10	35	4.85	37.07	4.81	35.92
11	37.64	10	35	1.23	29.46	1.09	27.28
12	20	1.18	35	3.59	52.17	3.45	49.84
13	20	18.82	35	3.12	34.59	3.08	33.60
14	20	10	8.54	5.17	26.1	5.10	23.04
15	20	10	61.46	3.21	15.37	3.09	15.17
16	20	10	35	6.06	44.89	6.02	43.45