Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 2;6:117–121. doi: 10.7150/jgen.28335

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Ivyspring International Publisher

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.

PMC Copyright notice

Isolation and PCR amplification of the V3/V4 16S rDNA region of endophytic bacterial isolates from Saccharum sp (A) Saccharum sp from Jamaica used for the isolation of endophytic bacteria. (B) Plating and isolation of bacteria on tryptic soy agar (TSA), Luria broth agar (LB), Reasoner's 2A agar (R2A), nutrient agar (N) and potato dextrose agar (PD). (C) 0.8% (w/v) agarose gel showing the resolution of the ~500bp amplicons of the V3/V4 16S rDNA region from the six endophytic bacteria. Lane 1-Marker (1Kb+), Lane 2-negative control (-DNA template), Lanes 3-8- V3/V4 amplicons from Pseudomonas sp RIT409, Pseudomonas sp RIT411, Pseudomonas sp RIT412, Pantoea sp RIT 413, Pseudocitrobacter sp RIT415, and Enterobacter sp RIT418 respectively.