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. 2018 Oct 3;8:14770. doi: 10.1038/s41598-018-32878-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative SEM images taken from cortex 3, 4 and corpus callosum. A well-perfused section from cortex 3 displays relatively round neuron somas (arrows in a), whereas dysmorphic and swollen neural somas are seen in poorly-perfused samples (arrowheads in b). Integrity of the intercellular tissue is also disrupted in poorly-perfused sections (b). Fine structure of blood vessels in cortex 4 is clearly discernable in well-perfused samples (c) unlike poorly-perfused samples (d). Neuronal fibers are well-preserved in well-perfused sections taken from corpus callosum (e), whereas intense electron discharges emerge as a result of poor perfusion (f). Scale bars: 20 µm.