Table 1.

Quantification of donut-like stained nuclei in the brain.

	Brain areas	NeuN* (%)	Hoechst* (%)	HMGB1* (%)	Nissl* (%)
Poorly-perfused brain	parietal cortex	46 ± 2	31 ± 3	41 ± 5	36 ± 5
	hind- and forelimb areas of cortex	43 ± 5	34 ± 4	39 ± 6	32 ± 4
	preoptic area (medial and lateral)	55 ± 4	34 ± 4	32 ± 5	36 ± 2
Well-perfused brain	parietal cortex	5 ± 1	1 ± 0	2 ± 1	5 ± 0
	hind- and forelimb areas of cortex	6 ± 1	2 ± 1	3 ± 1	6 ± 1
	preoptic area (medial and lateral)	3 ± 1	2 ± 0	3 ± 1	5 ± 1

In one subcortical and two cortical areas the average percentage of abnormally stained donut-like nuclei are given for poorly-perfused (6 mice) and well-perfused (4 mice) brains with different markers. For Nissl staining, the numbers refer to the ratio of dysmorphic neurons surrounded by swollen astrocyte end-feet to total cell nuclei in the contralateral hemisphere embedded in paraffin. NeuN staining gives a higher ratio because it shows the proportion of donut-stained neurons to total number of neurons unlike other markers, which give the ratio of donuts to the total nuclei including glial cells. Data are expressed as mean (percentage) ± standard error of mean (SEM). *p < 0.05 when poorly- and well-perfused groups compared. Brain anatomical areas are defined at the level of the anterior commissure according to Garcia et al.².