Table 4.
Primer sequences of the 18 enzymes related to pAp metabolism.
| NO | Gene | Primer Sequence (5′->3′) | NO | Gene | Primer Sequence (5′->3′) | ||
|---|---|---|---|---|---|---|---|
| 1 | AASDHPPT | F | CAGTGCTTGCTGCTGAACCTGAG | 10 | CHST12 | F | TCTTGTTCACTCCACTGCCTCTATC |
| R | GCTTCTGATTGTTTCCCATTCTTTG | R | ATTTTACTCCAGCATTCCCTTCC | ||||
| 2 | NDST2 | F | GTCCTCCGGGTATGAAGCTAAAA | 11 | CHST15 | F | GAGGACTGAAGGGAACGAAAACTG |
| R | CCTCACATCAAAGGTCAGCAAAG | R | CCGTAATGGAAAGGTGATGAGATC | ||||
| 3 | NDST4 | F | CAGGGACTGAAGAGGAAGATGAA | 12 | TPST1 | F | GGCATGTCTGGTGATTAGTTCTGT |
| R | ATGACTCGTTGTGGAAGAGGTGG | R | AGGTTTTGTTGGCTTTGAGGTCC | ||||
| 4 | HS3ST2 | F | CTGGAGTTTATCCGAGTACACCCG | 13 | TPST2 | F | ATGGAGGTAGGCAAGGAGAAGTG |
| R | GGAGCCTCTTGAGTGACAAAGTAGC | R | AAGTCGAGGATGAGCTTGAGTGA | ||||
| 5 | HS3ST4 | F | GCTCATCATCGGGGTCAAGAAAG | 14 | GAL3ST1 | F | TTGTTCGAGTCCTCCTTCCACTA |
| R | CATCCAAAGTCTTGGGCATCACA | R | GTCTTGCAGGAACTCGGTCAGC | ||||
| 6 | HS3ST5 | F | CTGCTTGAAATGCTGAACCTACA | 15 | PAPSS1 | F | AACAGATGCGGAAACATTACCAG |
| R | ACCACTCAATGCCCTTACCATAA | R | CACCTCCATCCAGAAGACAATCA | ||||
| 7 | HS6ST3 | F | TCTCACTGTGCTTGGCTTCATC | 16 | PAPSS2 | F | GAACCAGCAGAAATCCACCAATG |
| R | CTGTGGGCTTCACTATTTTCATCTA | R | CTCCTCCAGGGCAAAACTTATCG | ||||
| 8 | CHST3 | F | GGTTGGCTGTGCTGGTTACTGAT | 17 | IMPAD1 | F | ACAGGTCGCTCTTCAGACTTTTG |
| R | TGTGCCTTTATGATGCTTGTCTATG | R | TCTTGACTCTTATCAGGCACATCC | ||||
| 9 | CHST7 | F | CTTGCTACCAACAACTGCTGTGC | 18 | BPNT1 | F | ACGAGGGACCTGGGTCTACG |
| R | ACTTCATCTCAACCAATGCGTCC | R | GGAGGCTACCAACCGCATCA |