Table 2.
PCR primers used in the study.
| Primer | Sequence (5′ → 3′) | Purpose |
|---|---|---|
| PF-HindIII | CGGAAGCTTGGTCTATGATGCTGCAAGTC | yl-Hog1 deletion |
| PR-I-SceI | CTTATTACCCTGTTATCCCTATAAAGTCCGCCATGTTACG | yl-Hog1 deletion |
| TF-I-SceI | CAATAGGGATAACAGGGTAATCCAAACAATGTGGGTCAAAG | yl-Hog1 deletion |
| TR-NheI | CGGGCTAGCAGAATGGCGTGTTGAATATG | yl-Hog1 deletion |
| ptHog1-col-F | TTTGGTGCAGCAAGCACAAG | Evaluation of deletion |
| ptHog1-col-R | GATGGCGTGTCATCACATCG | Evaluation of deletion |
| Hog1-AscI-F | AATGGCGCGCCTAACATGGCGGACTTTATC | yl-Hog1 overexpression |
| Hog1-NheI-R | AGAGCTAGCACATTGTTTGGGTGTTTACTG | yl-Hog1 overexpression |
| col-Hog1-F | CTGAAGTACGTGCACTCTG | Evaluation of overexpression |
| col-Hog1-R | CTCGGTGATGATGGAGAAC | Evaluation of overexpression |
| TEF-seq-F | GTCAACTCACACCCGAAATC | Evaluation of overexpression |