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. 2018 Oct 3;8:14741. doi: 10.1038/s41598-018-33072-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotypic analysis of malate dehydrogenase activity. Malic enzymatic assays show that soluble extracts of A. baumannii strain A118 grown in LB, with or without HSA, have statistically significant reduced NADH formation under HSA induction. NADH formation, an indicator of malic dehydrogenase activity, was detected by relative fluorescence units (RFU) at an excitation spectrum of 340 nm and an emission spectrum of 460 nm.