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. 2018 Oct 3;8:14727. doi: 10.1038/s41598-018-32987-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differences in absolute abundances of OTUs detected at consecutive locations within the DWDS. OTU-level differences between DWDS locations (A–F) were calculated by dividing the number of 16S rRNA gene copies per OTU derived from two consecutive locations. For this, the medians of 16S rRNA gene copies per OTU that were measured over a 5-month period were used and their differences between two consecutive locations plotted using a binary logarithmic scale. OTUs with a relative abundance of <5% per sampled location are shown as grey dots, whereas OTUs with >5% relative abundance at location A (Peribacterales, Parcubacteria, and Gallionellaceae) are shown as green dots and OTUs with >5% relative abundance at location F (Comamonadaceae, Deferrisoma, and Nitrospira) are shown as blue dots. Differences with more than a 2-fold increase/decrease (dotted lines) were considered as significant differences that cannot be explained by technical variations introduced during sample processing.