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. 2018 Oct 3;8:14744. doi: 10.1038/s41598-018-33053-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Mucociliary clearance measured over ~10-min period after aerosolization of beads for 5 min. Data are from 21 mice. r = −0.305, p = 0.001 (MCC vs Time). (B) Mucociliary clearance measured over ~10-min period after aerosolization of beads for 15 min, 12 mice were studied. Data from 5 mice in which all beads were plaqued to the tracheal wall, thus exhibiting no MCC, are not plotted. r = −0.0130, p = 0.09. (C) Mean MCC rates from mice aerosolized for 5 or 15 min. When non-mobile rafts were observed, they were not included in the mean rate of MCC. However, if no mobile rafts were observed, as was the case for some of the mice receiving aerosol for 15 min (see below), the mean rate of MCC of these mice was included in the average as zero. In all studies, mean data were calculated by determining the average rate of MCC for each mouse and then averaging data for all mice. Mean data in all figures are +/− SEM. **p = 0.001.