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. 2018 Oct 3;8:14765. doi: 10.1038/s41598-018-32516-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Circadian rhythms of the adult SCN slices of Bmal1-Eluc:Per2-SLR2 mice (a) Sequential records of circadian Bmal1 (green) and Per2 (red) rhythm in an adult SCN slice. See also the legend for Fig. 1a. (b,c) A double plotted circadian Bmal1 (green) and Per2 (red) rhythm in a raster format. (d) Successive plots of circadian peak phases (Bmal1, green; Per2, red) and regressions lines fitted to them in the same SCN slice as used in (a). (e) Circadian periods of Bmal1 and Per2 rhythms in the cultured SCN (n = 8) calculated from a regression line. See also the legend for Fig. 1e. (f) Chi-square periodogram demonstrates significant but different circadian periods of Bmal1 (green) and Per2 (red) rhythm. See also the legend for Fig. 1f. (g) A phase difference (ψ) between the circadian Bmal1 and Per2 peaks in each cycle. See also the legend for Fig. 1g. (h) The mean and SD of ψ in the first and the last 10 cycles is indicated.