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. 2018 Oct 3;8:14765. doi: 10.1038/s41598-018-32516-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phase shifts of circadian Bmal1 and Per2 rhythm in the neonatal SCN slices in response to medium exchange (a) Representative records of phase shifts in response to medium exchange at 4 different phases. The circadian rhythms before and after medium exchange were illustrated for Bmal1 (green) and Per2 (red). See also the legend for Fig. 1a. The time of medium exchange was indicated with a vertical arrow. (b) Successive circadian peaks with regression lines fitted to them before and after medium exchange in the same SCN as used for (a). The abscissa indicates times of day as ZT and the ordinate days in culture. A square column in each panel indicates the time of medium exchange. A horizontal arrow indicates the direction and magnitude of phase-shifts calculated from two regression lines (see text).