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. 2018 Oct 3;8:14738. doi: 10.1038/s41598-018-32955-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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moxMaple3 excels for PALM microscopy of the ER. Distribution of localization precision for each molecule in cells expressing ER-mMaple3 (A) and ER-moxMaple3 (B). (C and D) Distributions of the localization precision and number of photons collected per molecule in cells expressing ER-mMaple3 and ER-moxMaple3. (E) Distribution of duration of molecular fluorescence (0.05 s frames) in cells expressing ER-mMaple3 (mean, 1.16; median, 1) and ER-moxMaple3 (mean 1.82, median, 1). Scale bar, 1 µm.