Fig. 3. Effects of MEG3 overexpression on EMT process.
Primary HSCs at Day 0 were transduced with Ad-MEG3 for 48 h. The mRNA (a) and protein (b) expressions of E-cadherin, BMP-7, Desmin and Vimenin were detected in MEG3 over-expressing HSCs (in vitro) and in the livers of CCl4 mice after Ad-MEG3 treatment (in vivo). c Immunofluorescence staining for Desmin (green), E-cadherin (red), and α-SMA (red) were evaluated by confocal laser microscopy. DAPI stained nuclei blue. Scale bar, 50 μm. d Cell migration was examined by wound healing. Dashed line indicates edge of cell migration. e Cell migration was evaluated by transwell migration assay. Five fields of migrated cells in the lower side of transwell were counted with a microscope at ×100. *P < 0.05