Figure 5.

Dynein accumulation at the spindle poles is induced by PYR-41. (a) Top: Cartoon illustrating inhibition of the ubiquitin E1 enzyme by PYR-41 and TAK-243. Bottom: Representative immunofluorescence images of stably expressed dynein heavy chain-GFP (DHC-GFP), ZW10, microtubules (DM1A) and DNA (Hoechst) after treating the cells for 15 min with the indicated compounds. Scale bar, 10 µm. (b) Representative images of live cells stably expressing dynein heavy chain-GFP (DHC-GFP) before and after addition of the indicated drugs at t = 0. Scale bar, 10 µm. (c) Representative immunofluorescence images of ZW10 in TS20 cells after treating the cells for 15 min with the indicated compounds. Scale bar, 10 µm.