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. 2018 Sep 27;6:123. doi: 10.3389/fcell.2018.00123

FIGURE 3.

FIGURE 3

Placental lineage analysis by RT-qPCR reveals differences in the normal repertoire of placental hormone gene expression between male and female Peg3 KO placenta. (A) RT-qPCR comparison of mature junctional zone markers Tpbpa and Flt1 at E14.5 in male and female Peg3 KO placenta relative to sex-matched WT placenta. (B) RT-qPCR comparison of markers exclusively or predominantly expressed in the mature spongiotrophoblast lineage. (C) RT-qPCR comparison of markers exclusively or predominantly expressed in the mature glycogen cell lineage. (D) RT-qPCR comparison of pan-TGC markers. (E) RT-qPCR comparison of markers of the mature labyrinth, syncytiotrophoblast layers I and II (SynT-I and SynT-II). (F) In situ hybridization of adjacent E14.5 placental sections with Prl8a8 and Psg17 riboprobes. Scale bars = 100 mm. For the RT-qPCR analysis, N = 4 placenta per genotype and sex, total N = 16 (2 vs. 2 from 2 independent litters, and 2 vs. 2 of each sex); error bars represent SEM. Statistical significance calculated from ANOVA with Bonferroni correction. ∗P < 0.05. Data in Supplementary Table S3 and at https://osf.io/jhc83/.