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. 2018 Sep 27;6:123. doi: 10.3389/fcell.2018.00123

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Copyright © 2018 Tunster, Boqué-Sastre, McNamara, Hunter, Creeth and John.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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No evidence for substantial relaxation of maternal silencing in female placenta. (A) RT-qPCR comparison of Peg3 expression between WT male and WT female placenta at E14.5. (B) RT-qPCR comparison of Peg3 expression between KO male and KO female placenta at E14.5. (C,D) High resolution image of three-plex analysis of junctional zone of male and female placenta inheriting the targeted Peg3 allele maternally (silenced). For the RT-qPCR analysis, N = 4 placenta per genotype (2 vs. 2 from 2 independent litters); error bars represent SEM. Statistical significance calculated using from ANOVA with Bonferroni correction. Data in Supplementary Table S4.