Table 1.
S. mutans strains and plasmids used in this study.
| Strain and plasmid | Relevant characteristics | Reference |
|---|---|---|
| S. mutans | ||
| XC | Wild type | Koga et al. (1989) |
| ΔgdpP-Int | S. mutans XC containing the IFDC2 cassette in place of gdpP | This study |
| ΔgdpP | S. mutans XC lacking gdpP without antibiotic cassettes | This study |
| ΔdhhP-Int | S. mutans XC containing t the IFDC2 cassette in place of dhhP | This study |
| ΔdhhP | S. mutans XC lacking dhhP without antibiotic cassettes | This study |
| ΔgdpPΔdhhP -Int | S. mutansΔgdpP containing t the IFDC2 cassette in place of dhhP | This study |
| ΔgdpPΔdhhP | S. mutans XC lacking gdpP and dhhP without antibiotic cassettes | This study |
| ΔgdpP-com | S. mutansΔgdpP containing pSM2140 | This study |
| ΔdhhP-com | S. mutansΔdhhP containing pSM1297 | This study |
| Plasmid | ||
| pMCL200 | CmR, cloning vector | Nakano et al. (1995) |
| pKOgdpP-Int | CmR and EmR; pMCL200 derivative containing the IFDC2 cassette flanked by the upstream and downstream regions of gdpP | This study |
| pKOgdpP | CmR; pMCL200 derivative containing the upstream and downstream regions of gdpP | This study |
| pKOdhhP-Int | CmR and EmR; pMCL200 derivative containing the IFDC2 cassette flanked by the upstream and downstream regions of dhhP | This study |
| pKOdhhP | CmR; pMCL200 derivative containing the upstream and downstream regions of dhhP | This study |
| pCold ProS2 | ApR; GST fusion expression vector | Takara Bio |
| pGdpP- ProS2 | ApR; Cold ProS2 derivative containing gdpP | This study |
| pDhhP- ProS2 | ApR; Cold ProS2 derivative containing dhhP | This study |
| pJY | KmR; a shuttle vector between Streptococcus and Escherichia | Yang et al. (2009) |
| pSM2140 | KmR; pJY derivative containing gdpP | This study |
| pSM1297 | KmR; pJY derivative containing dhhP | This study |