Strains |
|
|
L. monocytogenes strains |
HL06 |
Wild-type strain; serotype 1/2c |
Yu and Jiang, 2014 |
HL06CIP1 |
Ciprofloxacin-induced derivative of HL06 |
This study |
HL06CIP2 |
Ciprofloxacin-induced derivative of HL06 |
This study |
HL06CIP3 |
Ciprofloxacin-induced derivative of HL06 |
This study |
HL06CIP4 |
Ciprofloxacin-induced derivative of HL06 |
This study |
HL06CIP4Δlde
|
HL06CIP4 with deletion of lde
|
This study |
HL06CIP4ΔmdrL
|
HL06CIP4 with deletion of mdrL
|
This study |
HL06CIP4ΔlexA
|
HL06CIP4 with deletion of lexA
|
This study |
CHL06CIP4Δlde
|
Complemented strain of HL06CIP4Δlde
|
This study |
HL06CIP4ΔldepERL3 |
HL06CIP4Δlde containing pERL3 |
This study |
E. coli strains |
|
|
DH5α |
Chemical competent strain |
Biomed, Beijing, China |
DH10β |
Chemical competent strain |
Biomed, Beijing, China |
Plasmids |
|
|
pMAD |
Cloning shuttle integration vector with a thermosensitive origin of replication for low-GC-content Gram-positive bacteria, AmpR and EryR
|
Arnaud et al., 2004 |
pMAD-Δlde
|
pMAD containing homologous region up- and down-stream of HL06CIP4 lde
|
This study |
pMAD-ΔmdrL
|
pMAD containing homologous region up- and down-stream of HL06CIP4 mdrL
|
This study |
pMAD-ΔlexA
|
pMAD containing homologous region up- and down-stream of HL06CIP4 lexA
|
This study |
pERL3 |
Plasmid capable of replication in L. monocytogenes, EryR
|
Sibelius et al., 1996 |
plde
|
pERL3 containing 1,519bp of upstream nucleotides, coding sequence, and downstream terminator sequence of lde
|
This study |