Skip to main content
. 2018 Sep 27;9:2350. doi: 10.3389/fmicb.2018.02350

Table 1.

Bacterial strains and plasmids used in this study.

Strain or plasmid Genotype Reference or source
Strains
L. monocytogenes strains
HL06 Wild-type strain; serotype 1/2c Yu and Jiang, 2014
HL06CIP1 Ciprofloxacin-induced derivative of HL06 This study
HL06CIP2 Ciprofloxacin-induced derivative of HL06 This study
HL06CIP3 Ciprofloxacin-induced derivative of HL06 This study
HL06CIP4 Ciprofloxacin-induced derivative of HL06 This study
HL06CIP4Δlde HL06CIP4 with deletion of lde This study
HL06CIP4ΔmdrL HL06CIP4 with deletion of mdrL This study
HL06CIP4ΔlexA HL06CIP4 with deletion of lexA This study
CHL06CIP4Δlde Complemented strain of HL06CIP4Δlde This study
HL06CIP4ΔldepERL3 HL06CIP4Δlde containing pERL3 This study
E. coli strains
DH5α Chemical competent strain Biomed, Beijing, China
DH10β Chemical competent strain Biomed, Beijing, China
Plasmids
pMAD Cloning shuttle integration vector with a thermosensitive origin of replication for low-GC-content Gram-positive bacteria, AmpR and EryR Arnaud et al., 2004
pMAD-Δlde pMAD containing homologous region up- and down-stream of HL06CIP4 lde This study
pMAD-ΔmdrL pMAD containing homologous region up- and down-stream of HL06CIP4 mdrL This study
pMAD-ΔlexA pMAD containing homologous region up- and down-stream of HL06CIP4 lexA This study
pERL3 Plasmid capable of replication in L. monocytogenes, EryR Sibelius et al., 1996
plde pERL3 containing 1,519bp of upstream nucleotides, coding sequence, and downstream terminator sequence of lde This study