Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Genotype | Reference or source |
|---|---|---|
| Strains | ||
| L. monocytogenes strains | ||
| HL06 | Wild-type strain; serotype 1/2c | Yu and Jiang, 2014 |
| HL06CIP1 | Ciprofloxacin-induced derivative of HL06 | This study |
| HL06CIP2 | Ciprofloxacin-induced derivative of HL06 | This study |
| HL06CIP3 | Ciprofloxacin-induced derivative of HL06 | This study |
| HL06CIP4 | Ciprofloxacin-induced derivative of HL06 | This study |
| HL06CIP4Δlde | HL06CIP4 with deletion of lde | This study |
| HL06CIP4ΔmdrL | HL06CIP4 with deletion of mdrL | This study |
| HL06CIP4ΔlexA | HL06CIP4 with deletion of lexA | This study |
| CHL06CIP4Δlde | Complemented strain of HL06CIP4Δlde | This study |
| HL06CIP4ΔldepERL3 | HL06CIP4Δlde containing pERL3 | This study |
| E. coli strains | ||
| DH5α | Chemical competent strain | Biomed, Beijing, China |
| DH10β | Chemical competent strain | Biomed, Beijing, China |
| Plasmids | ||
| pMAD | Cloning shuttle integration vector with a thermosensitive origin of replication for low-GC-content Gram-positive bacteria, AmpR and EryR | Arnaud et al., 2004 |
| pMAD-Δlde | pMAD containing homologous region up- and down-stream of HL06CIP4 lde | This study |
| pMAD-ΔmdrL | pMAD containing homologous region up- and down-stream of HL06CIP4 mdrL | This study |
| pMAD-ΔlexA | pMAD containing homologous region up- and down-stream of HL06CIP4 lexA | This study |
| pERL3 | Plasmid capable of replication in L. monocytogenes, EryR | Sibelius et al., 1996 |
| plde | pERL3 containing 1,519bp of upstream nucleotides, coding sequence, and downstream terminator sequence of lde | This study |