Table 2.

Primers used in this study.

Gene	Primer	Sequence (5′-3′)	Reference
Primers for amplification of QRDRs
gyrA	GyrA-F	CGTGGAACCTCGTCGTAAA	This study
	GyrA-R	CCATAATCATCCCAGCAGTT
gyrB	GyrB-F	AAGCGCGCGCGTGAAGT	Godreuil et al., 2003
	GyrB-R	CGAGATTTAGAAACGTC
parC	ParC-F	AGGCGAAAGACATTTGAGT	This study
	ParC-R	TACGGTCAGTTTCATCACG
parE	ParE-F	GGAAAATTAACGCCAGC	Godreuil et al., 2003
	ParE-R	TCGGTCATGATAACTAC
Primers for RT-qPCR
lde	RTlde-F	GCGATGATTTTGATGGGA	This study
	RTlde-R	ACCGCTGCCGTTGATAGT
mdrL	RTmdrL-F	TAAAGTGAAAGAACCGAAGA	This study
	RTmdrL-R	CAAACATAATCCCCAAGC
16S rRNA	RT16S-F	GGGAGGCAGCAGTAGGGA	This study
	RT16S-R	CCGTCAAGGGACAAGCAG
Primers for mutant strain construction
Upstream of lde	lde-1	GCGTCGACTTTGGCACAGCATTAGGAT (SalI)	This study
	lde-2	GATAGAAGAATCTAGGTGGATTTTCTAATACAATTACCAGGAATAGGT
Downstream of lde	lde-3	ACCTATTCCTGGTAATTGTATTAGAAAATCCACCTAGATTCTTCTATC
	lde-4	CGACGCGTGACGATGGCTTGGTTCTG (MluI)
Upstream of mdrL	mdrL-1	CGGGATCCGTCCCTTGGTTCTGGCAT (BamHI)	This study
	mdrL-2	GTTGTAAGGTAAAATGTGCTGGAATACAACTACACTTCCCTTTCC
Downstream of mdrL	mdrL-3	GGAAAGGGAAGTGTAGTTGTATTCCAGCACATTTTACCTTACAAC
	mdrL-4	CGGAATTCTCCAATCATAAAGTTTCGTCAG (EcoRI)
Upstream of lexA	lexA-1	CGGGATCCGGACCTAAAATAACACCCAT (BamHI)	This study
	lexA-2	CCATGAAAATATCTAAACGCGGGCTTTATAGAGATATTCG
Downstream of lexA	lexA-3	CGAATATCTCTATAAAGCCCGCGTTTAGATATTTTCATGG
	lexA-4	CGACGCGTTGTTTTACTTTATGCGGAGT (MluI)
Primers for sequencing of the deletion area
lde	lde-5	TCCGTTTCCGCAACATAG	This study
	lde-6	GCACATTAGCCAATACCC
mdrL	mdrL-5	TGTAAAGCAGCAGGAGTG	This study
	mdrL-6	AAACGACGCTAATAACCAT
lexA	lexA-5	TCATTTGACTAAAAGGAAG	This study
	lexA-6	TTGAAGGTAAAGGGCTAA
Primers for complementation
lde	lde-7	CCGAGCTCATCGTGAACTTAATGGTTGG (SacI)	This study
	lde-8	CGGGATCCATCCTCATATAACTCAAGCG (BamHI)

Restriction sites are underlined. Overlapping areas are marked with italics.