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Expression cassette copy number and rProPG-1 expression level.
| PP-ProPG-1 clone | rProPG-1 (24 h) | rProPG-1 (72 h) | Copy # |
|---|---|---|---|
| Pro4 | - | + | 11 |
| Pro22 | + | +++ | 14 |
| Pro44 | ++ | +++ | 7 |
| Pro50 | - | - | 1 |
Supernatant samples at 24 and 72 h methanol induction from small-scale fermentations of different PP-ProPG-1 clones were assessed for rProPG-1 expression. (-) indicates no rProPG-1 was detected and (+), (++), (+++) indicate low, medium and high levels of rProPG-1 detected using dot blot analysis, respectively.
