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. 2018 Sep 27;9:2091. doi: 10.3389/fimmu.2018.02091

Figure 1.

Figure 1

Polyphyllin I does not affect apoptosis or proliferation of macrophages. (A) Chemical structure of Polyphyllin I (PPI) (CAS: 50773-41-6). (B–E) Cell apoptosis of PEMs and BMMs treated with PPI (1 μM) or the same volume DMSO determined by FITC-conjugated anti-Annexin V antibody and PE-propidium (PI) followed by flow cytometry. Data represent mean ± SEM of three pooled experiments. NS, P > 0.05, Student's t-test. (F) Cell growth of PEMs and BMMs treated with PPI (1 μM) or the same volume DMSO determined by CKK8 assay. Data represent mean ± SEM of four pooled experiments. NS, P > 0.05, Student's t-test. (G) Cell viability of Raw 264.7 cells treated with PPI (1 μM) or the same volume DMSO determined by CellTiter-Glo. Data represent mean ± SEM of four pooled experiments. NS, P > 0.05, Student's t-test.