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. 2018 Sep 27;9:2091. doi: 10.3389/fimmu.2018.02091

Figure 4.

Figure 4

PPI inhibited NF-κB luciferase activity. (A) NF-κB and Renilla luciferase plasmid were co-transfected into 293T cells. 8 h later, the cells were incubated with different doses of PPI (0, 0.25, 0.5, 1 μM) for 3 h, then treated with human TNF-α (20 ng/ml) for another 15 h, and the NF-κB and Renilla luciferase was detected by using Dual Luciferase® Reporter Gene Aassy Kit. Data represent mean ± SEM of three pooled experiments. *P < 0.05, one-way ANOVA. (B–F) The 293T cells, after transfection with MYD88, IRAK1, TRAF6, TAK1 or vector plasmids together with the NF-κB luciferase and Renilla plasmid for 8 h, was cultured with PPI (1 μM) for another 24 h, and the double luciferase was detected. Data represent mean ± SEM of three pooled experiments. NS, P > 0.05, *P < 0.05, one-way ANOVA. (G) Diagram illustrating the inhibitory effect of PPI on the TLR4 pathway.