Table 1.

Sequences of Primers Used in the Real-Time Polymerase Chain Reaction.

Genes	Sequences of primers	GenBank accession number	Annealing Tm(°C)	Product size (bp)
IL-1β	F:5′ CTGGTACATCAGCACCTCAC 3′ R:5′ AGAAACAGTCCAGCCCATAC 3′	NM_008361.4	55	124
IL-6	F: 5′ TGTATGAACAACGATGATGCACTT 3′ R: 5′ ACTCTGGCTTTGTCTTTCTTGTTATCT3′	NM_001314054.1	60	197
TNF-α	F: 5′ AGTGACAAGCCTGTAGCCC 3′: R:5′ GAGGTTGACTTTCTCCTGGTAT 3′	NM_013693.3	57	252
NOS2	F: 5′ GGAGTGACGGCAAACATGACT 3′ R: 5′ TCGATGCACAACTGGGTGAAC 3′	NM_001313922.1	52	127
p65	F: 5′ GATTGAAGAGAAGCGCAAAA 3′ R: 5′ CAGAAGTTGAGTTTCGGGTA 3′	NM_009045.4	55	131
CD40	F: 5′ TGTCATCTGTGAAAAGGTGGTC 3′ R: 5′ ACTGGAGCAGCGGTGTTATG 3′	NM_170702	60	120
CD86	F: 5′ TGTTTCCGTGGAGACGCAAG 3′ R: 5′ TTGAGCCTTTGTAAATGGGCA 3′	NM_170702	61	78
β-actin	F: 5′ CGTTGACATCCGTAAAGACC 3′ R: 5′ TAGGAGCCAGAGCAGTAATC 3′	NM_007393.5	56	110
hCCL5	F: 5′ CCAGCAGTCGTCTTTGTCAC 3′ R: 5′ CTCTGGGTTGGCACACACTT 3′	NM_002985	60	54
hCXCL10	F: 5′ GTGGCATTCAAGGAGTACCTC 3′ R: 5′ TGATGGCCTTCGATTCTGGATT 3′	NM_001565	60	198
hβ-actin	F: 5′ CATGTACGTTGCTATCCAGGC 3′ R: 5′ CTCCTTAATGTCACGCACGAT 3′	NM_001101	60	250

IL-1β, Mus musculus interleukin-1β; IL-6, Mus musculus interleukin-6; TNF-α, Mus musculus tumor necrosis factor α; NOS2, Mus musculus inducible nitric oxide synthase 2; CD40, Mus musculus CD40 antigen; CD86, Mus musculus CD86 antigen; hCXCL10, Homo sapiens chemokine (C-X-C motif) ligand 10; hCCL5: Homo sapiens chemokine (C-C motif) ligand 5; hβ-actin, homo beta actin. F, forward primer; R, reverse primer.