MOT versus duration of the previous closed event for 1, 10, and 50 μM cytosolic [Ca2+] and for high (left) and low (right) luminal [Ca2+]. The thick lines are the mean times of the data, and the shaded areas are the 95% confidence intervals (see Supporting Materials and Methods). The thin lines are fits of a sum of three exponentials, and the arrows indicate when these fits approximately reach steady state (i.e., 4τ3, as defined in Fig. 3). Fig. S10A shows the same for 200 and 1000 μM. Fig. S10B shows mean closed time versus open duration. To see this figure in color, go online.