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. 2018 Sep 27;12:319. doi: 10.3389/fncel.2018.00319

Figure 3.

Figure 3

Astrocytic glutamate release upon MOR activation is mediated by TREK-1. (A) Left, schematic diagram of sniffer-patch with acutely dissociated astrocytes from pSicoR-TREK-1-shRNA-mCherry-injected hippocampus and GluR1LY-GFP-expressing HEK cells. Right, differential interference contrast image and fluorescent image of GluR1LY-GFP-expressing HEK cell (green) and TREK-1-shRNA-mCherry-infected hippocampal astrocyte (red). (B) Representative traces of Ca2+ response and inward current in acutely dissociated hippocampal astrocyte of scrambled-shRNA and TREK-1-shRNA-injected mouse. (C) Summary bar graph for DAMGO-induced glutamate current normalized by the full activation current in scrambled shRNA and TREK-1-shRNA-injected mice. Data from at least three independent mice for each group. Unpaired two-tailed t-test with Welch’s correction (*P < 0.05).