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. 2018 Sep 27;12:319. doi: 10.3389/fncel.2018.00319

Figure 4.

Figure 4

MOR and TREK-1 are co-expressed in hippocampal astrocytes of MOR-mCherry mouse. (A) A structured illumination microscopic (SIM) image of a hippocampal astrocyte of MOR-mCherry mouse, immunostained with antibodies against GFAP and TREK-1. (B) 2D histogram of mCherry intensity and TREK-1 intensity in the SIM image (A). Pearson’s coefficient (r) is calculated within the colocalized pixels which were automatically thresholded by Imaris 9.2 program. (C) Representative confocal images of co-localization of MOR-mCherry and TREK-1 in astrocytes in hippocampal cornu ammonis 1 (CA1) of MOR-mCherry mice. (D) An example of ROI generation within a single astrocyte. (E) Representative 2D histogram of mCherry intensity and TREK-1 intensity in the confocal image. (F) Pearson’s coefficients (r) between mCherry and TREK-1 from confocal images of single astrocytes. (G) A confocal image of hippocampal tissues from WT and TREK-1 KO mice, stained with an antibody against TREK-1.