Figure 7. Sedimentation Velocity Analysis of rHBcAg and rHBeAg in Complex with Fab e21.

Raw absorbance scans of the (A) rHBcAg-Fab e21 and (B) rHBeAg-Fab e21 complexes, both at pH 7.5 and 250 mM NaCl. All analyses were performed at 40,000 rpm. The red arrow indicates the direction of sedimentation from top (meniscus) to bottom of the analytical cells. Despite being under assembly conditions the rHBcAg-Fab e21 and rHBeAg-Fab e21 complexes remain soluble, with sedimentation coefficient (s) of ~ 5.7 and 5.6, respectively. See also Figure S7 and Figure S8.