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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Mol Cancer Res. 2018 Jul 10;16(10):1470–1482. doi: 10.1158/1541-7786.MCR-18-0322

Figure 6. Point-mutation of nucleotides 333–336 or 349–352 of LINC00152 shows the importance of M8 hairpin for stimulating invasion.

Figure 6

A) Invasion of U87 cells after the different LINC00152 deletion mutants are overexpressed. Mut A or mut B are incapable of inducing invasion in U87 cells. Combining the two mutants (mut AB) restores the hairpin and induces invasion to the same level as full length LINC00152; * p-value < 0.05. Pictures were adjusted by +20% in brightness and +40% in contrast. B) qRT-PCR confirming overexpression of the different LINC00152 constructs. C) Predicted secondary structure of full length LINC00152 with the black line marking the sequence at the tip of the hairpin and the light grey and dark grey lines marking the residues that are mutated in Mut A or B, respectively. D) Predicted secondary structures of LINC00152 mutants A, B and AB. The black, light grey and dark grey lines mark the corresponding residues as in Fig. 6C.