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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Microcirculation. 2018 Aug 27;25(7):e12492. doi: 10.1111/micc.12492

Figure 2.

Figure 2.

Insulin-mediated eNOS phosphorylation is regulated by PI3K/Akt pathways. LECs were treated with insulin (100nM) and S961(100nM), as described in the Materials and Methods, and representative western blots are shown: (A) Akt (Ser473) phosphorylation, (B) Akt (Thr308) phosphorylation, and (C) eNOS (Ser1177) phosphorylation. The relative expression of p-Akt (Ser473)/Akt, p-Akt (Thr308)/Akt, and p-eNOS/eNOS was quantified and plotted (n=3/group). Data represent mean ± SEM. #p<0.05 vs. Control; $p<0.05 vs. insulin. LECs were treated with insulin (100nM) and (D) LY294002 (20μg/ml) or (E) Akt inhibitor XI (20μM), as described in the Materials and Methods, and representative western blots are shown. The p-eNOS/eNOS expression was quantified and plotted (n=3/group). Data represent mean fold changes ± SEM. #p<0.05 vs. control; $p<0.05 vs. insulin.