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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: J Allergy Clin Immunol. 2018 Apr 3;143(1):229–244.e9. doi: 10.1016/j.jaci.2018.03.007

Figure 7. ILC217s concomitantly express IL-5 and IL-13 but express few GM-CSF.

Figure 7

(A) Lung lymphocytes were isolated from wild-type mice treated with IL-33. (D and E) Lung lymphocytes were isolated from wild-type and Rag1−/− mice at the steady-state (SS), after treated with IL-33 for 4 days(IL-33) or the “rested” state (Rested). (A, D and E) The expression of IL-5, IL-13, GM-CSF and IL-17 gated on ILC2 (Lin− GATA3+) was analyzed by flow cytometry. (B) Genes with differential expression from ILC217 (Lin−ST2+GFP+) and IL-17− ILC2 (Lin−ST2+GFP−) with more than 1.5 folds in all 4 biological repeats were shown. Ratio of gene expression level of ILC217/IL-17− ILC2 was calculated and the Log1.5 value of the ratio was shown. (C) mRNA expression of GM-CSF was analyzed by real-time RT-PCR. Each line connected one pair of ILC217 and IL-17− ILC2 from one biological repeat. Statistical analysis was performed using paired Student’s t test. (E) Percentages of GM-CSF+ILC217 (upper right quadrant in D) and GM-CSF+IL17− ILC2 (upper left quadrant in D) in ILC2 from indicated groups were shown. Data are means+SEM. (A to E) Data are representative of two independent experiments.