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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: J Neurosurg Anesthesiol. 2019 Apr;31(2):247–256. doi: 10.1097/ANA.0000000000000499

Figure 1. Experimental Protocol.

Figure 1

Sixteen rats were randomly separated to receive either unrestricted access to a 4% BCAA solution dissolved in water (n=8) or a plain water solution (n=8) ad libitum. Following 10 days of consumption, rats were implanted with the MSO pump, and seizure activity was monitored thereafter for a period of 21 days. For the duration of the EEG recording period, MSO was continuously infused and rats were maintained on the BCAA or plain water solution. After this monitoring period, rats were perfused and the brains were harvested for immunohistochemical analysis. BCAA concentrations were analyzed in venous blood samples that were drawn at two time points during the experiment. Three rats from the BCAA group were excluded from the final analysis after postmortem histological analysis confirmed that the MSO pump was outside of dentate gyrus.