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. 2018 Sep 19;15(146):20180406. doi: 10.1098/rsif.2018.0406

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© 2018 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 1. — Genetic regulation of the adhesive phenotype via ag43 expression. Cells containing accessory plasmid pL31N and either sfGFP labelled pSEG4ag or mCherry labelled pSEG5ag exhibited inducible clumping with (b) 1 mM IPTG unlike (a) without IPTG in liquid culture due to ag43 induction. (c, top panel) Overnight cultures with pSEG4ag or pSEG5ag and pL31N with (+) 1 mM IPTG or (−) 0 IPTG were left to stand (bottom panel) unshaken for 5 h, displayed autoaggregation. Microcolonies grown on agar pads showed significant morphological changes with adhesion in (d) pSEG4ag and pL31N without IPTG, (e) pSEG4ag and pL31N with 1 mM IPTG, (f) strongly adhesive plasmid pKAYag with 100 mM arabinose. CellModeller colonies with adhesive interactions show similar colony morphology when compared with micrographs at adhesion strengths (g) 0, (h) 0.05 and (i) 10 in simulation units. Scale bars, 10 μm.