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. 2018 Sep 19;15(146):20180406. doi: 10.1098/rsif.2018.0406

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© 2018 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 2. — The artificial patterning system was composed of segregating plasmids (a) pSEG4s, (b) pSEG5s, (c) pSEG4ag, (d) pSEG5ag and (e) accessory plasmid pL31N. The segregating plasmids were controllably compatible, and were combined into single cells and made to segregate to generate two-domain colonies with variable adhesive properties: (f) pSEG4s and pSEG5s, (g) pSEG4ag and pSEG5s, (h) pSEG4s and pSEG5ag and (i) pSEG4ag and pSEG5ag. (a–i) Scale bar, 100 μm.