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PVT1 induces EMT and enhances stem cell-like properties of SCCHN in vitro. (A) qPCR and (B) western blotting assays were performed to check the expression of EMT markers including E-cadherin and Vimentin. (C) Tumorsphere formation assays were used to determine cancer stem cell-like properties after PVT1 overexpression. **, P < 0.01; ***, P < 0.001. Vector, FaDu cells transfected with empty lentivirus; cDNA, FaDu cells transfected by lentivirus mediated PVT1 cDNA.
