Figure 5.

Wnt/β-catenin signaling pathway is involved in PVT1- mediated SCCHN progression. Specific inhibitor iCRT14 (20μmol/L) was used to impede Wnt/β-catenin signaling pathway in FaDu cells infected with PVT1 cDNA and empty vector, and then: (A) western blotting assays were used to check alterations of Wnt/β-catenin signaling molecules. (B) Cell proliferation was assayed by CCK8. (C) Transwell migration and (D) invasion assays were used to examine the changes of migration and invasion. (E) E-cadherin and (F) Vimentin mRNAs were examined by qPCR.