Figure 2.

Overview of the NLC Formulation for RNA Delivery

(A) Schematic representation of rvRNA surface adsorbed to NLC nanoparticle post-admixture. The following physical and biophysical properties of NLC_v1 and CNE were compared: (B) intensity weighted size distribution, (C) particle size (Z-average diameter) evolution over 9 months to evaluate colloidal stability of formulations stored at 25°C, (D) protection from RNase, and (E) in vitro SEAP expression (relative luminescence units, RLUs) as a function of the molar ratio of formulation nitrogen (N) to RNA phosphate (P) values.