Table 1.

Exemplary Formulation Compositions

Formulation	DOTAP (%w/v)	Span XX (%w/v)	Tween 80 (%w/v)	Squalene (%w/v)	Dynasan 114 (%w/v)	S:O ratioa	Z-average (nm) (PDI)	Zeta Potential (mV)
NLCv1	0.4	Span 60: 0.5	0.5	4.75	0.25	0.18	91.90 (0.17)	15.60 ± 0.12
NLCv2	3.0	Span 60: 3.7	3.7	3.75	0.25	1.68	40.55 (0.20)	28.4 ± 1.27
CNE	0.4	Span 85: 0.5	0.5	4.3	0.00	0.14	97.23 (0.06)	13.1 ± 3.25

^a$a-S:O\left(molarratio\right)=\frac{\left[DOTAP\right]+\left[Span\right]+\left[Tween\right]}{\left[Squalene+Dynasan114\right]}$