Table 2.
Calculated Papp for wild-type and knockdown/knockout MDCK cell populations
| Cell Line | Papp, (cm/s) × 106 | P Value vs. MDCK |
|---|---|---|
| MDCK | 0.029847 (SD 0.008143) (n = 17) | |
| Occludin knockdown | 0.028890 (SD 0.007807) (n = 3) | 0.858954 |
| ZO-1 knockdown | 0.091359 (SD 0.025161) (n = 8) | 0.000173 |
| TOCA-1 knockout | 0.057063 (SD 0.015859) (n = 3) | 0.009557 |
Data are means (SD) of multiple independent experiments (numbers in parentheses). Calcein flux rates for individual experiments were averaged for each cell line. Apparent permeability (Papp) was calculated according to Van Itallie et al. (29). Significance (P values) was determined using two-tailed Student’s t-test compared with the flux rate for wild-type Madin-Darby canine kidney (MDCK) cell populations. ZO-1, zonula occludens-1.