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. 2018 May 23;120(3):998–1009. doi: 10.1152/jn.00783.2017

Fig. 5.

Fig. 5.

Adenosine released from astrocytes upon protease-activated receptor-1 (PAR1) activation requires activation of D1-like dopamine receptors (D1LRs) to modulate locomotor-related activity. A: raw (top) and rectified/integrated (bottom) traces recorded from left (L) and right (R) L2 ventral roots showing the effect of the PAR1 agonist TFLLR-NH2 (TFLLR; 10 µM) on locomotor-related activity induced by 5-hydroxytryptamine (5-HT; 10 µM) and N-methyl-d-aspartate (NMDA; 5 µM). Bi: locomotor burst frequency over 5 min during a control period, upon a 5-min application of TFLLR, and during a 25-min washout. Individual data points are shown in gray, and means are represented by black lines. n = 7 preparations. Bii: time course plot of normalized data aggregated into 1-min bins showing no change in burst frequency upon TFLLR application. n = 7. Ci: locomotor burst amplitude over 5 min during a control period, upon a 5-min application of TFLLR, and during a 25-min washout. n = 7. a.u., Arbitrary units. Cii: time course plot of normalized data aggregated into 1-min bins showing no change in burst amplitude upon TFLLR application. n = 7. D: raw (top) and rectified/integrated (bottom) traces recorded from LL2 and RL2 ventral roots showing the effect of TFLLR on locomotor-related activity induced by 5-HT and NMDA in the presence of the selective agonist of D1LRs SKF 38393 (100 nM). Ei: locomotor burst frequency over 5 min during a control period, upon a 5-min application of TFLLR, and during a 25-min washout. SKF 38393 was present throughout. n = 11. Eii: time course plot of normalized data aggregated into 1-min bins showing a transient reduction in burst frequency upon TFLLR application in the presence of SKF 38393. n = 11. Fi: locomotor burst amplitude over 5 min during a control period, upon a 5-min application of TFLLR, and during a 25-min washout. SKF 38393 was present throughout. n = 11. Fii: time course plot of normalized data aggregated into 1-min bins showing no change in burst amplitude upon TFLLR application in the presence of SKF 38393. n = 11. Error bars show ±SE. Statistically significant difference: **P < 0.01.