Fig. 2.

Morphological changes, proliferation, migration, and phagocytosis of microglia after stimulation by growth factors. (A) Cultured primary microglia on day 1 and day 3 after activation by GM-CSF or SCF. Scale Bar = 50 μm. (B) Cell number of microglia (cells/mm²) on day 1 and day 3 after activation by GM-CSF or SCF. “Basal” showed cell counts of microglia at the time of dissemination. Bars showed means + SD. *p < 0.05 (as compared to) day 1 in each group. **p < 0.05 as compared to day 1 control. ^#p < 0.05. (C) Migration assay after stimulation of microglia by growth factors. Numbers of microglia were counted 12 hours after the scratching test, after 3 days pre-incubation with GM-CSF or SCF. Bars show means + SD. *p < 0.05. (D) Phagocytosis assay after stimulation of microglia by growth factors. Percentages of FITC-positive microglia were calculated for each group. Bars showed means + SD. *p < 0.05.