Figure 2.
Patient 2: A) Leukemic promyelocytes in the bone marrow (Wright-Giemsa, 1000x). B) Flow cytometry dot plot showing co expression of myeloid (CD33) and T-lymphoid (cCD3) antigens; the blue dotted line and arrow indicate the dimmer expression of cCD3 in leukemic cells as compared with normal residual T-cells. C) MPO, immunoperoxidase, 2000x..D) TdT, immunoperoxidase, 2000x. E) CD2, immunoperoxidase, 2000x. F) CD3, immunoperoxidase, 2000x. G) CD5, immunoperoxidase, 2000x. H) CD7, immunoperoxidase, 2000x H. Note in E and F that the expression of C and CD3 is dimmer in leukemic cells in contrast to the background normal T-cells. Only normal T-cells are positive for CD5 and CD7.
