. Author manuscript; available in PMC: 2019 Sep 1.

Published in final edited form as: J Hematop. 2018 Aug 9;11(3):67–74. doi: 10.1007/s12308-018-0329-z

Table 2:

Distribution of positive and negative different antigens by flow cytometry and immunohistochemistry

	Flow cytometry			Immunohistochemistry
	CD45 vs. SSC	Positive markers	Negative markers	Immunohistochemistry
Patient 1	84.2% with dim CD45 and intermediate side scatter	CD34,CD117(p), CD33,CD13,CD11c,CD38, CD71, cMPO, HLA-DR(p), CD7(p), cCD3,and TdT(p).	CD11b, CD14, CD15, CD16, CD56, CD64, surface CD3, CD2,CD4,CD5, CD8,CD10, CD19, CD20, cCD79a	Blasts express MPO, CD34, CD117, CD2^*(dim, subset), CD3 (dim), CD7 (dim), and TdT (subset, dim). Blasts negative for CD5.
Patient 2	89% with dim CD45 and intermediate side scatter	CD34(p), CD117, CD33, CD13, CD38, CD64, CD71, MPO(dim), HLA-DR (p), CD2, and cCD3	CD11b,CD11c, CD14, CD15, CD16, CD56, surface CD3, CD4, CD5, CD7, CD8, CD10, CD19, CD20, cCD79a, TdT	Blasts express MPO, CD117, CD34 (focal), CD2, CD3 (subset, dim), TdT^*(subset, dim). Blasts negative for CD5 and CD7.

These antigens were dim positive by immunohistochemistry, but negative by flow cytometry.